2BB0

Structure of Imidazolonepropionase from Bacillus subtilis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE BW7A
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	BW7A
Temperature [K]	100
Detector technology	CCD
Collection date	2004-06-02
Detector	MARRESEARCH
Wavelength(s)	0.980
Spacegroup name	P 1 21 1
Unit cell lengths	57.730, 106.340, 66.470
Unit cell angles	90.00, 89.92, 90.00

Refinement procedure

Resolution	20.000 - 2.000
R-factor	0.191
Rwork	0.188
R-free	0.22100
Structure solution method	SAD
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHELX
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.030
High resolution limit [Å]	2.000	2.000
Rmerge	0.098	0.256
Number of reflections	54728
Completeness [%]	99.8	99.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	289	20% PEG4000, 0.1M Tris-HCl, 0.2M Sodium Acetate, 2% benzamidine hydrochloride, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K