2BAP
Crystal structure of the N-terminal mDia1 Armadillo Repeat Region and Dimerisation Domain in complex with the mDia1 autoregulatory domain (DAD)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-05-28 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.95 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 138.450, 138.450, 210.890 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.980 - 3.300 |
| R-factor | 0.292 |
| Rwork | 0.288 |
| R-free | 0.36400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1z2c |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.248 |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 3.400 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Rmerge | 0.141 | |
| Number of reflections | 18490 | 0 |
| <I/σ(I)> | 16.79 | |
| Completeness [%] | 81.9 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.1 | 293 | 3.7 M NaFormiate pH 7.1, 100 mM HEPES pH 7.1, 4% (w/v) PEG5000-MME, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
