2BAM
RESTRICTION ENDONUCLEASE BAMHI COMPLEX WITH DNA AND CALCIUM IONS (PRE-REACTIVE COMPLEX).
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 110 |
| Detector technology | IMAGE PLATE |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 105.200, 79.400, 66.400 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.000 |
| R-factor | 0.233 |
| Rwork | 0.233 |
| R-free | 0.25100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1bhm |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.500 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | |
| High resolution limit [Å] | 2.000 | |
| Rmerge | 0.074 | 0.310 * |
| Total number of observations | 93202 * | |
| Number of reflections | 31554 | |
| <I/σ(I)> | 17.35 | |
| Completeness [%] | 81.6 | 61 * |
| Redundancy | 2.95 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 5.3 | 20 * | 12-14% PEG8000, 0.1M MES (PH 5.3), 0.15M KCL, 5% GLYCEROL |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 20-25 (mg/ml) | |
| 10 | 1 | reservoir | 0.15 (M) | ||
| 11 | 1 | reservoir | MES | 0.1 (M) | pH5.3 |
| 12 | 1 | reservoir | glycerol | 5 (%) | |
| 2 | 1 | drop | 0.5 (M) | ||
| 3 | 1 | drop | potassium phosphate | 20 (mM) | pH6.9 |
| 4 | 1 | drop | dithiothreitol | 1 (mM) | |
| 5 | 1 | drop | glycerol | 10 (%) | |
| 6 | 1 | drop | DNA | 10 (mg/ml) | |
| 7 | 1 | drop | PEG8000 | 12-14 (%) | |
| 8 | 1 | drop | MES | 0.1 (M) | pH5.3 |
| 9 | 1 | reservoir | PEG8000 | 12-14 (%) |
