2B9L

Crystal structure of prophenoloxidase activating factor-II from the beetle Holotrichia diomphalia

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NW12A
Synchrotron site	Photon Factory
Beamline	AR-NW12A
Temperature [K]	100
Detector technology	CCD
Collection date	2005-03-15
Detector	MARRESEARCH
Wavelength(s)	0.9496
Spacegroup name	C 1 2 1
Unit cell lengths	106.780, 76.587, 69.833
Unit cell angles	90.00, 113.45, 90.00

Refinement procedure

Resolution	33.630 - 2.000
R-factor	0.209
Rwork	0.209
R-free	0.23800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1cgi
RMSD bond length	0.006
RMSD bond angle	1.300
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.630	2.070
High resolution limit [Å]	2.000	2.000
Number of reflections	31743
Completeness [%]	90.8	76.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	287	0.15M ammonium sulfate, 1.25M lithium sulfate, 0.1M sodium citrate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 287K