2B9L
Crystal structure of prophenoloxidase activating factor-II from the beetle Holotrichia diomphalia
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
Synchrotron site | Photon Factory |
Beamline | AR-NW12A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-03-15 |
Detector | MARRESEARCH |
Wavelength(s) | 0.9496 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 106.780, 76.587, 69.833 |
Unit cell angles | 90.00, 113.45, 90.00 |
Refinement procedure
Resolution | 33.630 - 2.000 |
R-factor | 0.209 |
Rwork | 0.209 |
R-free | 0.23800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1cgi |
RMSD bond length | 0.006 |
RMSD bond angle | 1.300 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 33.630 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Number of reflections | 31743 | |
Completeness [%] | 90.8 | 76.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 287 | 0.15M ammonium sulfate, 1.25M lithium sulfate, 0.1M sodium citrate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 287K |