2B9B
Structure of the Parainfluenza Virus 5 F Protein in its Metastable, Pre-fusion Conformation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 32-ID |
| Synchrotron site | APS |
| Beamline | 32-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-03-31 |
| Detector | MAR CCD 165 mm |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 160.206, 259.864, 154.012 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.150 - 2.850 |
| R-factor | 0.222 |
| Rwork | 0.222 |
| R-free | 0.25900 |
| Structure solution method | MIRAS |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.500 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHARP |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.150 | 3.000 |
| High resolution limit [Å] | 2.850 | 2.850 |
| Rmerge | 0.073 | 0.376 |
| Number of reflections | 74802 | |
| <I/σ(I)> | 7.1 | 2 |
| Completeness [%] | 99.7 | 99.7 |
| Redundancy | 7.6 | 7.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 295 | sodium potassium tartrate, imidazole, pH 8.00, temperature 295K |
