2B97
Ultra-high resolution structure of hydrophobin HFBII
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE BW7A |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | BW7A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-04-24 |
Detector | MARRESEARCH |
Wavelength(s) | 0.7747 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 78.544, 46.254, 34.687 |
Unit cell angles | 90.00, 111.64, 90.00 |
Refinement procedure
Resolution | 20.000 - 0.750 |
Rwork | 0.130 |
R-free | 0.14820 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 1r2m |
RMSD bond length | 0.027 |
RMSD bond angle | 0.066 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHELX |
Refinement software | SHELXL-97 |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 0.760 |
High resolution limit [Å] | 0.750 | 0.750 |
Number of reflections | 132282 | |
<I/σ(I)> | 18.01 | 1.91 |
Completeness [%] | 98.7 | 91.3 |
Redundancy | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.39 | 298 | 0.2 M lithium sulfate, 15% PEG2000, 0.1 M sodium HEPES pH 7.5, manganese dichloride 50mM , pH 7.39, VAPOR DIFFUSION, HANGING DROP, temperature 298K |