2B97
Ultra-high resolution structure of hydrophobin HFBII
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7A |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-04-24 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.7747 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 78.544, 46.254, 34.687 |
| Unit cell angles | 90.00, 111.64, 90.00 |
Refinement procedure
| Resolution | 20.000 - 0.750 |
| Rwork | 0.130 |
| R-free | 0.14820 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1r2m |
| RMSD bond length | 0.027 |
| RMSD bond angle | 0.066 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELX |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 0.760 |
| High resolution limit [Å] | 0.750 | 0.750 |
| Number of reflections | 132282 | |
| <I/σ(I)> | 18.01 | 1.91 |
| Completeness [%] | 98.7 | 91.3 |
| Redundancy | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.39 | 298 | 0.2 M lithium sulfate, 15% PEG2000, 0.1 M sodium HEPES pH 7.5, manganese dichloride 50mM , pH 7.39, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
