2B8Z
Crystal structure of the interleukin-4 variant R85A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 93 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-08-21 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 91.190, 91.190, 45.770 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.500 |
| R-factor | 0.21352 |
| Rwork | 0.211 |
| R-free | 0.26368 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1hik |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.400 |
| Data reduction software | CrystalClear ((MSC/RIGAKU)) |
| Data scaling software | CrystalClear ((MSC/RIGAKU)) |
| Phasing software | CNS |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.300 | 2.430 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmerge | 0.056 | 0.265 |
| Number of reflections | 8375 | |
| <I/σ(I)> | 17.3 | 4.6 |
| Completeness [%] | 98.9 | 99.6 |
| Redundancy | 4.4 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 294 | 52% Ammonium sulfate, 0.1M Sodium citrate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
