2B8Y
Crystal structure of the interleukin-4 variant T13DF82D
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-4 |
Synchrotron site | ESRF |
Beamline | ID14-4 |
Temperature [K] | 93 |
Detector technology | CCD |
Collection date | 2003-05-21 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.9500 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 91.032, 91.032, 46.026 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 14.790 - 1.800 |
R-factor | 0.21768 |
Rwork | 0.216 |
R-free | 0.24080 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1hik |
RMSD bond length | 0.011 |
RMSD bond angle | 1.194 |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | CNS |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.500 | 1.900 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.071 | 0.211 |
Number of reflections | 18359 | |
<I/σ(I)> | 15.2 | 4.9 |
Completeness [%] | 99.6 | 100 |
Redundancy | 5.7 | 4.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 294 | 52% Ammonium sulfate, 0.1M Sodium citrate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 294K |