2B8Y
Crystal structure of the interleukin-4 variant T13DF82D
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2003-05-21 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9500 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 91.032, 91.032, 46.026 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 14.790 - 1.800 |
| R-factor | 0.21768 |
| Rwork | 0.216 |
| R-free | 0.24080 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1hik |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.194 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | CNS |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.500 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.071 | 0.211 |
| Number of reflections | 18359 | |
| <I/σ(I)> | 15.2 | 4.9 |
| Completeness [%] | 99.6 | 100 |
| Redundancy | 5.7 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 294 | 52% Ammonium sulfate, 0.1M Sodium citrate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
