2B8X
Crystal structure of the interleukin-4 variant F82D
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2002-08-17 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9183 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 90.968, 90.968, 46.086 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.700 |
| R-factor | 0.2276 |
| Rwork | 0.227 |
| R-free | 0.24811 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1hik |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.322 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | CNS |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.700 | 1.690 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.080 | 0.291 |
| Number of reflections | 26060 | |
| <I/σ(I)> | 26.6 | 5.7 |
| Completeness [%] | 100.0 | 99.9 |
| Redundancy | 14.4 | 9.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 52% Ammonium sulfate, 0.1M Sodium citrate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
