2B8M
Crystal structure of a rmlc-like cupin family protein with a double-stranded beta-helix fold (mj0764) from methanocaldococcus jannaschii at 1.70 A resolution
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-09-09 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9796, 0.9797, 1.0000 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 49.830, 49.830, 78.740 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.080 - 1.700 |
| R-factor | 0.15197 |
| Rwork | 0.149 |
| R-free | 0.20400 |
| Structure solution method | MAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.441 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 29.080 | 29.080 | 1.760 |
| High resolution limit [Å] | 1.700 | 3.660 | 1.700 |
| Rmerge | 0.091 | 0.527 | 0.527 |
| Number of reflections | 12267 | 2358 | 2106 |
| <I/σ(I)> | 7.84 | 17.45 | 2.23 |
| Completeness [%] | 99.3 | 97.2 | |
| Redundancy | 3.48 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | 277 | 0.2M (NH4)2SO4, 20.0% PEG-3350, No Buffer, VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 277K, pH 6 |
