2B8I

Crystal Structure and Functional Studies Reveal that PAS Factor from Vibrio vulnificus is a Novel Member of the Saposin-Fold Family

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Temperature [K]	100
Detector technology	CCD
Collection date	2002-12-20
Detector	ADSC QUANTUM 4
Spacegroup name	F 2 2 2
Unit cell lengths	57.157, 75.192, 80.206
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.000 - 1.800
R-factor	0.2
Rwork	0.199
R-free	0.23200
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	REFMAC (5.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.860
High resolution limit [Å]	1.800	1.800
Number of reflections	7767
Completeness [%]	96.0	92

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	294	0.1M Tris-HCl pH 8.5, 22% (w/v) PEG 4000, 0.2M MgCl2, VAPOR DIFFUSION, HANGING DROP, temperature 294K