2B7X
Sequential reorganization of beta-sheet topology by insertion of a single strand
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 110 |
Detector technology | CCD |
Collection date | 1998-11-06 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 61.380, 78.080, 143.350 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 3.000 |
R-factor | 0.241 |
Rwork | 0.239 |
R-free | 0.31910 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3lzm |
RMSD bond length | 0.025 |
RMSD bond angle | 2.100 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 3.250 |
High resolution limit [Å] | 3.000 | 3.000 |
Rmerge | 0.065 | |
Number of reflections | 10387 | |
<I/σ(I)> | 7.4 | |
Completeness [%] | 72.3 | 66.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.8 | 298 | Polyehtylene glycol 4000, 50mM Ammonium sulfate, , pH 7.8, VAPOR DIFFUSION, HANGING DROP, temperature 298K |