2B7R
Structure of E378D mutant flavocytochrome c3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7B |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-09-09 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.87 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 45.961, 92.879, 79.486 |
| Unit cell angles | 90.00, 91.17, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.700 |
| R-factor | 0.15768 |
| Rwork | 0.156 |
| R-free | 0.18034 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1qjd |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.314 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0003) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.067 | 0.129 |
| Number of reflections | 72178 | |
| <I/σ(I)> | 18.7 | 6.9 |
| Completeness [%] | 98.4 | 94.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 277 | 0.1M TrisHCl, 80mM NaCl, 10mM Na fumarate, 15% PEG8000, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
