2B7P
Crystal structure of quinolinic acid phosphoribosyltransferase from Helicobacter pylori with phthalic acid
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 6B |
| Synchrotron site | PAL/PLS |
| Beamline | 6B |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-08-22 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9794, 0.9796, 0.9718 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 149.593, 149.593, 146.246 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.510 |
| R-factor | 0.228 |
| Rwork | 0.228 |
| R-free | 0.26000 |
| Structure solution method | MAD |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.800 |
| Data reduction software | AUTOMAR |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.080 | 2.580 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Number of reflections | 40644 | |
| Completeness [%] | 96.4 | 96.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 297 | 0.1M HEPES-NaOH, 1.5M Li2SO4, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 297K |
