2B7M

Crystal Structure of the S. cerevisiae Exocyst Component Exo70p

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	CCD
Collection date	2004-12-05
Detector	ADSC QUANTUM 210
Wavelength(s)	0.97950, 0.97963, 0.9393
Spacegroup name	C 1 2 1
Unit cell lengths	415.729, 55.129, 132.930
Unit cell angles	90.00, 104.05, 90.00

Refinement procedure

Resolution	20.000 - 3.500
R-factor	0.254
Rwork	0.254
R-free	0.30500
Structure solution method	MAD
RMSD bond length	0.007
RMSD bond angle	1.200
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	3.620
High resolution limit [Å]	3.500	3.500
Number of reflections	36080
<I/σ(I)>	12.6
Completeness [%]	95.0	92
Redundancy	2.7	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.1	295	0.1 M MES pH 6.1, 20 % PEG 20000, 0.2 M magnesium acetate, VAPOR DIFFUSION, HANGING DROP, temperature 295K