2B7M
Crystal Structure of the S. cerevisiae Exocyst Component Exo70p
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-12-05 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.97950, 0.97963, 0.9393 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 415.729, 55.129, 132.930 |
| Unit cell angles | 90.00, 104.05, 90.00 |
Refinement procedure
| Resolution | 20.000 - 3.500 |
| R-factor | 0.254 |
| Rwork | 0.254 |
| R-free | 0.30500 |
| Structure solution method | MAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.200 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 3.620 |
| High resolution limit [Å] | 3.500 | 3.500 |
| Number of reflections | 36080 | |
| <I/σ(I)> | 12.6 | |
| Completeness [%] | 95.0 | 92 |
| Redundancy | 2.7 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.1 | 295 | 0.1 M MES pH 6.1, 20 % PEG 20000, 0.2 M magnesium acetate, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
