2B78
A putative sam-dependent methyltransferase from Streptococcus mutans
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | ENRAF-NONIUS FR591 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-03-17 |
Detector | BRUKER SMART 6000 |
Wavelength(s) | 1.5418 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 168.472, 50.659, 53.963 |
Unit cell angles | 90.00, 104.22, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.000 |
Rwork | 0.241 |
R-free | 0.25900 |
Structure solution method | SIRAS |
Data reduction software | PROTEUM PLUS |
Data scaling software | PROTEUM PLUS |
Phasing software | SOLVE |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.910 |
High resolution limit [Å] | 1.800 | 1.800 |
Number of reflections | 52534 | |
<I/σ(I)> | 11 | 2.77 |
Completeness [%] | 94.5 | 74.1 |
Redundancy | 4.54 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 289 | PEG8000, Sodium Cacodylate,Magnesium Acetates, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 289 | PEG8000, Sodium Cacodylate,Magnesium Acetates, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K |