2B71
Plasmodium yoelii cyclophilin-like protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-09-13 |
| Detector | RIGAKU RAXIS IV |
| Spacegroup name | P 41 3 2 |
| Unit cell lengths | 132.399, 132.399, 132.399 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.800 - 2.500 |
| R-factor | 0.19218 |
| Rwork | 0.190 |
| R-free | 0.24247 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1xyh |
| RMSD bond length | 0.023 |
| RMSD bond angle | 2.003 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.800 | 2.540 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.137 | 0.842 |
| Number of reflections | 14318 | |
| <I/σ(I)> | 6.1 | 5.4 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 38.8 | 39.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 296 | 3.2 M NaCl, 0.1 M Bis-Tris propane, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 296K |
