2B70

T4 Lysozyme mutant L99A at ambient pressure

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE F1
Synchrotron site	CHESS
Beamline	F1
Temperature [K]	293
Detector technology	CCD
Collection date	2004-11-15
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9174
Spacegroup name	P 32 2 1
Unit cell lengths	60.956, 60.956, 96.597
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	52.780 - 2.400
R-factor	0.15352
Rwork	0.150
R-free	0.21790
RMSD bond length	0.021
RMSD bond angle	1.647
Data scaling software	SCALEPACK
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	52.780	2.462
High resolution limit [Å]	2.400	2.400
Rmerge	0.073
Number of reflections	7969
Completeness [%]	98.1	99.35

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.1	293	~ 2.0 M Na/K phosphate, 50 mM beta-mercaptoethanol, pH 7.1, VAPOR DIFFUSION, HANGING DROP, temperature 293K