2B6X

T4 Lysozyme mutant L99A at 200 MPa

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE F1
Synchrotron site	CHESS
Beamline	F1
Temperature [K]	293
Detector technology	CCD
Collection date	2004-11-15
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9174
Spacegroup name	P 32 2 1
Unit cell lengths	60.594, 60.594, 95.646
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	52.490 - 2.107
R-factor	0.163
Rwork	0.161
R-free	0.20400
RMSD bond length	0.016
RMSD bond angle	1.394
Data scaling software	SCALEPACK
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	52.490	2.161
High resolution limit [Å]	2.107	2.107
Rmerge	0.036
Number of reflections	11485
Completeness [%]	94.8	88.07

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.1	293	~ 2.0 M Na/K phosphate, 50 mM beta-mercaptoethanol, pH 7.1, VAPOR DIFFUSION, HANGING DROP, temperature 293K