2B6E
X-Ray Crystal Structure of Protein HI1161 from Haemophilus influenzae. Northeast Structural Genomics Consortium Target IR63.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-07-12 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 60.651, 102.878, 101.364 |
| Unit cell angles | 90.00, 98.36, 90.00 |
Refinement procedure
| Resolution | 29.970 - 1.900 |
| R-factor | 0.191 |
| Rwork | 0.191 |
| R-free | 0.22000 |
| Structure solution method | SAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.300 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.063 | 0.335 |
| Number of reflections | 188231 | |
| <I/σ(I)> | 18.3 | 3.4 |
| Completeness [%] | 98.9 | 98.2 |
| Redundancy | 3.5 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 293 | 0.1 Sodium Acetate, 20% PEG-4000, 2% benzamidine, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
