2B65
Crystal structure of the complex of C-lobe of bovine lactoferrin with maltose at 1.5A resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X31 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X31 |
Temperature [K] | 203 |
Detector technology | CCD |
Collection date | 2005-09-20 |
Detector | MARRESEARCH |
Wavelength(s) | 0.803 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 61.484, 49.846, 65.161 |
Unit cell angles | 90.00, 107.07, 90.00 |
Refinement procedure
Resolution | 20.000 - 1.500 |
R-factor | 0.194 |
Rwork | 0.193 |
R-free | 0.21862 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1nkx |
RMSD bond length | 0.012 |
RMSD bond angle | 1.744 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.530 |
High resolution limit [Å] | 1.500 | 1.500 |
Number of reflections | 60932 | |
<I/σ(I)> | 10 | 2.2 |
Completeness [%] | 90.4 | 99.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 6.5 | 298 | 0.1M MES, 25% POLYETHYLENE GLYCOL MONOMETHYL ETHER 550, 0.01M ZINC SULPHATE, pH 6.5, VAPOR DIFFUSION, temperature 298K |