2B63
Complete RNA Polymerase II-RNA inhibitor complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-01-22 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9189 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 224.585, 399.815, 286.727 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 3.800 |
| R-factor | 0.273 |
| Rwork | 0.252 |
| R-free | 0.27300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1wcm |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.900 |
| High resolution limit [Å] | 3.800 | 3.800 |
| Rmerge | 0.367 | |
| Number of reflections | 248494 | |
| <I/σ(I)> | 3.2 | |
| Completeness [%] | 99.9 | 99.7 |
| Redundancy | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 200 mM ammonium acetate, 150 mM magnesium acetate, 50 mM Hepes (pH 7.0), 5% PEG 6000, and 5 mM TCEP |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | ammonium acetate | ||
| 2 | 1 | 1 | magnesium acetate | ||
| 3 | 1 | 1 | Hepes | ||
| 4 | 1 | 1 | PEG 6000 | ||
| 5 | 1 | 1 | TCEP | ||
| 6 | 1 | 1 | H2O | ||
| 7 | 1 | 2 | ammonium acetate | ||
| 8 | 1 | 2 | magnesium acetate | ||
| 9 | 1 | 2 | PEG 6000 |
