2B5V
Crystal structure of glucose dehydrogenase from Haloferax mediterranei
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-06-23 |
| Detector | MARRESEARCH |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 61.791, 110.225, 152.332 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.000 |
| Rwork | 0.161 |
| R-free | 0.20700 |
| Structure solution method | MIR |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.062 | 0.309 |
| Number of reflections | 32706 | |
| <I/σ(I)> | 15.99 | 3.05 |
| Completeness [%] | 92.0 | 95.8 |
| Redundancy | 4.8 | 2.42 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 290 | pH 7.00, Temperature 290 K, Vapor diffusion, Hanging drop, VAPOR DIFFUSION, HANGING DROP |
