2B5H
1.5 A Resolution Crystal Structure of Recombinant R. Norvegicus Cysteine Dioxygenase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12B |
| Synchrotron site | NSLS |
| Beamline | X12B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-03-03 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 57.553, 57.553, 123.070 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.780 - 1.500 |
| R-factor | 0.182 |
| Rwork | 0.182 |
| R-free | 0.20800 |
| Structure solution method | SAD |
| RMSD bond length | 0.016 |
| RMSD bond angle | 2.100 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.800 | 1.539 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.046 | 0.318 |
| Number of reflections | 33389 | |
| <I/σ(I)> | 52.4 | 7 |
| Completeness [%] | 97.2 | 93.84 |
| Redundancy | 13.1 | 13.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 298 | 0.2 M ammonium acetate plus 0.1 M tri-sodium citrate, pH 5.6, with 26% (w/v) polyethylene glycol 4000, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
