2B5F

Crystal structure of the spinach aquaporin SoPIP2;1 in an open conformation to 3.9 resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-2
Synchrotron site	ESRF
Beamline	ID14-2
Temperature [K]	100
Detector technology	CCD
Collection date	2004-02-09
Detector	ADSC QUANTUM 4
Wavelength(s)	0.931
Spacegroup name	P 21 21 2
Unit cell lengths	181.300, 103.970, 67.070
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 3.900
R-factor	0.31
Rwork	0.290
R-free	0.33200
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	1.990
RMSD bond angle	0.024
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	4.100
High resolution limit [Å]	3.900	3.900
Number of reflections	10862
Completeness [%]	91.1	92.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1