2B5D
Crystal structure of the novel alpha-amylase AmyC from Thermotoga maritima
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-04-04 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.12710, 0.97972, 0.97997, 0.98393 |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 112.164, 112.164, 335.544 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.200 |
| R-factor | 0.22139 |
| Rwork | 0.219 |
| R-free | 0.25709 |
| Structure solution method | MAD |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.698 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0013) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Number of reflections | 50618 | |
| <I/σ(I)> | 19.1 | |
| Completeness [%] | 92.4 | 60.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298 | 4M Na-formiate, 5% isopropanol, 2mM DTT, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
