2B5A

C.BclI, Control Element of the BclI Restriction-Modification System

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL7-1
Synchrotron site	SSRL
Beamline	BL7-1
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2002-03-27
Detector	MARRESEARCH
Wavelength(s)	1.08
Spacegroup name	P 21 21 2
Unit cell lengths	63.205, 65.016, 73.296
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.741 - 1.543
R-factor	0.167
Rwork	0.165
R-free	0.20050
Structure solution method	MAD
RMSD bond length	0.012
RMSD bond angle	1.409
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MLPHARE
Refinement software	REFMAC (refmac_5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	99.000	1.560
High resolution limit [Å]	1.540	1.540
Rmerge	0.051	0.457
Number of reflections	44934
<I/σ(I)>	38	3.2
Completeness [%]	98.3	68.4
Redundancy	5.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	298	25% (w/v) Polyethylene glyco 400, 25 mM ammonium sulfate, 50 mM citrate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K
1	VAPOR DIFFUSION, HANGING DROP	6.5	298	25% (w/v) Polyethylene glyco 400, 25 mM ammonium sulfate, 50 mM citrate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K
1	VAPOR DIFFUSION, HANGING DROP	6.5	298	25% (w/v) Polyethylene glyco 400, 25 mM ammonium sulfate, 50 mM citrate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K