2B57
Guanine Riboswitch C74U mutant bound to 2,6-diaminopurine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-03-18 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 131.900, 34.800, 41.460 |
| Unit cell angles | 90.00, 90.83, 90.00 |
Refinement procedure
| Resolution | 19.860 - 2.150 |
| R-factor | 0.246 |
| Rwork | 0.228 |
| R-free | 0.28900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.200 |
| Data scaling software | d*TREK |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.230 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Number of reflections | 10387 | |
| Completeness [%] | 99.1 | 90.71 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 20% PEG 2K, 480 mM Ammonium Acetate, 12 mM Cobalt Hexammine, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG 2K | ||
| 2 | 1 | 1 | Ammonium Acetate | ||
| 3 | 1 | 1 | Cobalt Hexammine | ||
| 4 | 1 | 1 | H2O | ||
| 5 | 1 | 2 | PEG 2K | ||
| 6 | 1 | 2 | Ammonium Acetate | ||
| 7 | 1 | 2 | Cobalt Hexammine |
