2B50
Human Nuclear Receptor-Ligand Complex 2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM14 |
Synchrotron site | ESRF |
Beamline | BM14 |
Temperature [K] | 65 |
Detector technology | CCD |
Detector | ADSC QUANTUM 4r |
Wavelength(s) | 0.939261 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 39.776, 92.084, 95.017 |
Unit cell angles | 90.00, 97.00, 90.00 |
Refinement procedure
Resolution | 94.490 - 2.000 |
R-factor | 0.199 |
Rwork | 0.197 |
R-free | 0.24900 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.011 |
RMSD bond angle | 1.329 |
Data scaling software | CCP4 ((SCALA)) |
Refinement software | REFMAC (5.2.0005) |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | vapour diffusion, hanging drop | 8.5 | 291 | 5% PEG 8000, 200mM KCl, 40mM bis-tris-propane pH 8.5, 2.5% 1,2-propanediol, 0.5% n-heptyl-B-D-glucopyranoside, 1mM EDTA, 10mM DTT, vapour diffusion, hanging drop, temperature 291K |