2B4Z

Crystal structure of cytochrome C from bovine heart at 1.5 A resolution.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X6A
Synchrotron site	NSLS
Beamline	X6A
Detector technology	CCD
Collection date	2005-07-18
Detector	ADSC QUANTUM 210
Wavelength(s)	1.03320
Spacegroup name	P 21 21 21
Unit cell lengths	36.840, 54.480, 55.330
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.520 - 1.500
R-factor	0.196
Rwork	0.196
R-free	0.21800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1crc
RMSD bond length	0.005
RMSD bond angle	1.300
Data reduction software	HKL-2000
Data scaling software	CCP4 ((TRUNCATE))
Phasing software	CNS
Refinement software	CNS (1.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	38.000
High resolution limit [Å]	1.500
Number of reflections	17207

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROSEEDING	7	277	PEG 1000, SODIUM PHOSPHATE, pH 7.0, MICROSEEDING, temperature 277K