2B4O
Structure of the R258K mutant of Selenomonas ruminantium PTP-like phytase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS FR591 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-02-16 |
| Detector | Nonius Kappa CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 45.837, 137.187, 79.954 |
| Unit cell angles | 90.00, 103.53, 90.00 |
Refinement procedure
| Resolution | 40.000 - 2.300 |
| Rwork | 0.191 |
| R-free | 0.22600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.038 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.200 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.078 | 0.315 |
| Number of reflections | 39348 | |
| Completeness [%] | 92.1 | 83.1 |
| Redundancy | 4.3 | 1.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.5 | 295 | 1.35 M ammonium sulfate, 0.90 M NaCl, 1 % 1,6 hexandiol, pH 6.5, vapor diffusion, temperature 295K |
