2B4M
Crystal structure of the binding protein OpuAC in complex with proline betaine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MPG/DESY, HAMBURG BEAMLINE BW6 |
| Synchrotron site | MPG/DESY, HAMBURG |
| Beamline | BW6 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.05 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 88.562, 28.318, 102.826 |
| Unit cell angles | 90.00, 93.87, 90.00 |
Refinement procedure
| Resolution | 15.000 - 2.800 |
| Rwork | 0.231 |
| R-free | 0.28302 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2b4l |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.133 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0000) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 2.840 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Number of reflections | 10846 | |
| <I/σ(I)> | 2.4 | |
| Completeness [%] | 83.0 | 70.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.25 | 274 | Tris, ammonium acetate, PEG 4000, pH 8.25, VAPOR DIFFUSION, HANGING DROP, temperature 274K |
