2B4F

Structure Of A Cold-Adapted Family 8 Xylanase in complex with substrate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE BW7A
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	BW7A
Temperature [K]	100
Detector technology	CCD
Collection date	2005-05-04
Detector	MARRESEARCH
Wavelength(s)	0.94
Spacegroup name	P 21 21 21
Unit cell lengths	50.711, 91.099, 99.633
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 1.950
R-factor	0.14811
Rwork	0.146
R-free	0.17850
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	: 1H13
RMSD bond length	0.011
RMSD bond angle	1.287
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	REFMAC (5.1.24)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.010
High resolution limit [Å]	1.950	1.950
Number of reflections	34436
Completeness [%]	100.0	100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	277	PEG8000, sodium cacodylate, ammonium acetate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 277K