2B4E
Crystal Structure of Murine Coronin-1: monoclinic form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-02-25 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 51.358, 80.284, 52.889 |
| Unit cell angles | 90.00, 111.92, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.300 |
| R-factor | 0.15905 |
| Rwork | 0.155 |
| R-free | 0.22867 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2aq5 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.448 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.420 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Number of reflections | 16388 | |
| <I/σ(I)> | 8.7 | 2.1 |
| Completeness [%] | 92.6 | 87.3 |
| Redundancy | 3.6 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 292 | 200 mM ammonium acetate, 100 mM Bis-Tris PH 5.5, 25% PEG 3350, VAPOR DIFFUSION, SITTING DROP, temperature 292K |
