2B4A

Crystal structure of a response regulator receiver domain protein (bh3024) from bacillus halodurans c-125 at 2.42 A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	CCD
Collection date	2005-06-15
Detector	ADSC QUANTUM 315
Spacegroup name	P 41 21 2
Unit cell lengths	39.003, 39.003, 172.741
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.940 - 2.420
Rwork	0.182
R-free	0.24300
Structure solution method	MR
Starting model (for MR)	1kgs
RMSD bond length	0.017
RMSD bond angle	1.732
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	REFMAC (5.2.0013)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	28.940	28.940	2.420
High resolution limit [Å]	2.360	10.550	2.360
Rmerge	0.086	0.038	0.710
Number of reflections	5994
<I/σ(I)>	6	13.5	1.1
Completeness [%]	98.0
Redundancy	10.9	7.8	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION,SITTING DROP,NANODROP	6.2	277	0.2M NaCl, 10.0% PEG-8000, 0.1M Na, K-Phosphate, pH 6.2, VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 277K