2B49
Crystal Structure of the Catalytic Domain of Protein Tyrosine Phosphatase, non-receptor Type 3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-08-05 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.00800 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 62.675, 61.242, 75.885 |
| Unit cell angles | 90.00, 101.21, 90.00 |
Refinement procedure
| Resolution | 40.000 - 1.540 |
| R-factor | 0.20048 |
| Rwork | 0.199 |
| R-free | 0.23014 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1L8K.pdb |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.402 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.390 | 1.620 |
| High resolution limit [Å] | 1.540 | 1.540 |
| Number of reflections | 41752 | |
| Completeness [%] | 99.7 | 99.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | Ammonium Sulphate, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
