2B44
Truncated S. aureus LytM, P 32 2 1 crystal form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MPG/DESY, HAMBURG BEAMLINE BW6 |
| Synchrotron site | MPG/DESY, HAMBURG |
| Beamline | BW6 |
| Detector technology | CCD |
| Collection date | 2004-11-23 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.05 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 100.434, 100.434, 103.038 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.250 - 1.830 |
| R-factor | 0.18131 |
| Rwork | 0.181 |
| R-free | 0.19313 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1qwy |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.592 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.860 |
| High resolution limit [Å] | 1.830 | 1.830 |
| Rmerge | 0.063 | 0.244 |
| Number of reflections | 51861 | |
| <I/σ(I)> | 27.6 | 4.7 |
| Completeness [%] | 97.6 | 96 |
| Redundancy | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 294 | 0.1 M Tris, 2.0 M monoammonium dihydrogen phosphate, pH 4.5, VAPOR DIFFUSION, SITTING DROP, temperature 294K |
