2B3B
Thermus thermophilus Glucose/Galactose Binding Protein With Bound Glucose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-03-16 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.97917 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 121.643, 134.874, 159.547 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 1.950 |
R-factor | 0.186 |
Rwork | 0.185 |
R-free | 0.22500 |
Structure solution method | SAD |
RMSD bond length | 0.012 |
RMSD bond angle | 1.346 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHELXL-97 |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.020 |
High resolution limit [Å] | 1.950 | 1.950 |
Rmerge | 0.086 | 0.455 |
Number of reflections | 181610 | |
<I/σ(I)> | 2.4 | |
Completeness [%] | 95.6 | 82.4 |
Redundancy | 5.4 | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 290 | 10% (w/v) Isopropanol, 0.1M Sodium Citrate pH5.6, 10% PEG 4000, VAPOR DIFFUSION, HANGING DROP, temperature 290K |