2B34

Structure of MAR1 Ribonuclease from Caenorhabditis elegans

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU300
Temperature [K]	103
Detector technology	IMAGE PLATE
Collection date	2005-03-01
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	89.545, 113.973, 151.322
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.670 - 2.141
R-factor	0.2217
Rwork	0.220
R-free	0.26270
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	The starting model was created by the Swiss-Model Server based on PDB entry 1X9G using the C. elegans target sequence (F35G2.2)
RMSD bond length	0.007
RMSD bond angle	1.058
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	REFMAC (5.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.220
High resolution limit [Å]	2.140	2.140
Rmerge	0.062	0.168
Number of reflections	84631
<I/σ(I)>	23.4
Completeness [%]	98.3	94.7
Redundancy	3.5	3.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	291	20% PEG3350, 0.2M sodium acetate, 0.1M Tris , pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K