2B2F

Ammonium Transporter Amt-1 from A.fulgidus (Native)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE BW7A
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	BW7A
Temperature [K]	100
Detector technology	CCD
Collection date	2005-03-07
Detector	MARRESEARCH
Wavelength(s)	1.08
Spacegroup name	H 3
Unit cell lengths	111.308, 111.308, 136.563
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	35.200 - 1.720
R-factor	0.1807
Rwork	0.181
R-free	0.19970
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1u77
RMSD bond length	0.006
RMSD bond angle	1.298
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000	1.750
High resolution limit [Å]	1.720	1.720
Number of reflections	67039
Completeness [%]	98.4	90.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	298	PEG 400, Tris/HCl, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K