2B2E
RNA stemloop from bacteriophage MS2 complexed with an N87S,E89K mutant MS2 capsid
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX14.2 |
| Synchrotron site | SRS |
| Beamline | PX14.2 |
| Temperature [K] | 298 |
| Detector technology | CCD |
| Collection date | 2003-05-10 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.978 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 288.062, 288.062, 652.812 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 3.150 |
| R-factor | 0.227 |
| Rwork | 0.227 |
| R-free | 0.22900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ms2 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.400 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.360 |
| High resolution limit [Å] | 3.150 | 3.150 |
| Number of reflections | 128885 | |
| <I/σ(I)> | 2.9 | 1.4 |
| Completeness [%] | 72.1 | 72.4 |
| Redundancy | 1.9 | 1.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 303 | MS2 COAT PROTEIN IN 1.25% or 1.5% PEG 8000, 0.4M SODIUM PHOSPHATE BUFFER, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 303K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG 8000 | ||
| 2 | 1 | 1 | 0.4M SODIUM PHOSPHATE BUFFER | ||
| 3 | 1 | 2 | PEG 8000 | ||
| 4 | 1 | 2 | 0.4M SODIUM PHOSPHATE BUFFER |
