2B2D

RNA stemloop operator from bacteriophage QBETA complexed with an N87S,E89K mutant MS2 capsid

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX14.2
Synchrotron site	SRS
Beamline	PX14.2
Temperature [K]	298
Detector technology	CCD
Collection date	2003-05-10
Detector	ADSC QUANTUM 4
Wavelength(s)	0.978
Spacegroup name	H 3 2
Unit cell lengths	287.800, 287.800, 652.600
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.000 - 2.900
R-factor	0.197
Rwork	0.197
R-free	0.20300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2ms2
RMSD bond angle	0.010
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.990
High resolution limit [Å]	2.900	2.900
Number of reflections	147903
<I/σ(I)>	2.8	1.6
Completeness [%]	64.8	65.2
Redundancy	2.1	1.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.4	303	MS2 Coat protein in 1.25% or 1.5% PEG 8000, 0.4M SODIUM PHOSPHATE BUFFER, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 303K

Crystallization Reagents

ID	crystal ID	solution ID	reagent name	concentration	details
1	1	1	PEG 8000
2	1	1	0.4M SODIUM PHOSPHATE BUFFER
3	1	1	water
4	1	2	PEG 8000
5	1	2	0.4M SODIUM PHOSPHATE BUFFER
6	1	2	water