2B1Y
Crystal Structure of Protein of Unknown Function ATU1913 from Agrobacterium tumefaciens str. C58
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-08-16 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9795 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 40.428, 99.646, 55.827 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.810 - 1.800 |
| R-factor | 0.2129 |
| Rwork | 0.212 |
| R-free | 0.22070 |
| Structure solution method | SAD |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.557 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.054 | 0.169 |
| Number of reflections | 10811 | |
| <I/σ(I)> | 16.9 | 9 |
| Completeness [%] | 93.8 | 100 |
| Redundancy | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 298 | 2M NH4Sulphate,5% iso-Propanol, 2Mm K/Na Tartrate, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
