2B1L

Crystal structure of N-terminal 57 residue deletion mutant of E. coli CcmG protein(residues 58-185)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BSRF BEAMLINE 3W1A
Synchrotron site	BSRF
Beamline	3W1A
Temperature [K]	293
Detector technology	CCD
Collection date	2005-03-10
Detector	MARRESEARCH
Wavelength(s)	1.0000
Spacegroup name	P 1 21 1
Unit cell lengths	37.691, 43.349, 78.986
Unit cell angles	90.00, 94.94, 90.00

Refinement procedure

Resolution	29.140 - 1.900
R-factor	0.193
Rwork	0.193
R-free	0.23100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	structure of E.coli CcmG
RMSD bond length	0.005
RMSD bond angle	1.200
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.970
High resolution limit [Å]	1.900	1.900
Rmerge	0.053	0.233
Number of reflections	19846
Completeness [%]	98.1	85.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Seeding	7.8	293	HEPES buffer, ammonium sulfate, PEG 4000, pH 7.8, Seeding, temperature 293.0K