2B1J
Crystal Structure of Unphosphorylated CheY Bound to the N-Terminus of FliM
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 298 |
Detector technology | IMAGE PLATE |
Collection date | 2005-01-24 |
Detector | RIGAKU RAXIS II |
Wavelength(s) | 1.542 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 40.040, 62.160, 54.223 |
Unit cell angles | 90.00, 96.77, 90.00 |
Refinement procedure
Resolution | 40.700 - 2.400 |
R-factor | 0.187 |
Rwork | 0.181 |
R-free | 0.24000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3chy |
RMSD bond length | 0.007 |
RMSD bond angle | 1.300 |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | EPMR |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.700 | 2.490 |
High resolution limit [Å] | 2.400 | 2.400 |
Number of reflections | 10174 | |
<I/σ(I)> | 18.8 | |
Completeness [%] | 97.3 | 96 |
Redundancy | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 277 | PEG 6000, magnesium chloride, MES buffer, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |