2B1H
Crystal structure analysis of anti-HIV-1 V3 Fab 2219 in complex with UG29 peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.1 |
| Synchrotron site | ALS |
| Beamline | 5.0.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-06-07 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 62.899, 94.501, 96.617 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 67.420 - 2.000 |
| R-factor | 0.20646 |
| Rwork | 0.205 |
| R-free | 0.23837 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2b0s |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.728 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 67.420 | 2.030 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 39609 | |
| <I/σ(I)> | 19.7 | 2.6 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 4.8 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 25% MPEG 5000, 0.2M disodium tartrate, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
