2B1E

The structures of exocyst subunit Exo70p and the Exo84p C-terminal domains reveal a common motif

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 8-BM
Synchrotron site	APS
Beamline	8-BM
Temperature [K]	200
Detector technology	CCD
Collection date	2005-02-18
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9792, 0.9794, 0.9611
Spacegroup name	P 21 21 21
Unit cell lengths	45.846, 60.605, 223.131
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.000
R-factor	0.223
Rwork	0.221
R-free	0.25000
Structure solution method	MAD
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.070
High resolution limit [Å]	2.000	2.000
Number of reflections	39711
Completeness [%]	99.4	96.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	277	PEG 3000, lithium sulfate, sodium cloride, dithiothreitol, L-cysteine, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K