2B18
N-terminal GAF domain of transcriptional pleiotropic repressor CodY.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX10.1 |
| Synchrotron site | SRS |
| Beamline | PX10.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-03-11 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.98000 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 34.696, 86.081, 54.819 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.800 |
| R-factor | 0.1564 |
| Rwork | 0.154 |
| R-free | 0.21234 |
| Structure solution method | SAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.341 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | HKL2Map |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.730 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.061 | 0.270 |
| Number of reflections | 16841 | |
| <I/σ(I)> | 20.2 | 1.7 |
| Completeness [%] | 88.6 | 29.2 |
| Redundancy | 3.6 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291 | PEG 5000, calcium acetate, isoleucine, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
