2B11

Crystal structure of the protein-protein complex between F82W cytochrome c and cytochrome c peroxidase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X25
Synchrotron site	NSLS
Beamline	X25
Temperature [K]	95
Detector technology	CCD
Collection date	2004-04-21
Detector	ADSC QUANTUM 315
Wavelength(s)	1.1
Spacegroup name	P 1 21 1
Unit cell lengths	44.884, 117.864, 88.470
Unit cell angles	90.00, 104.23, 90.00

Refinement procedure

Resolution	30.000 - 2.300
R-factor	0.267
Rwork	0.265
R-free	0.29300
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	2.380
High resolution limit [Å]	2.300	4.950	2.300
Rmerge	0.136	0.106	0.308
Number of reflections	37320
Completeness [%]	95.5
Redundancy	2.7	2.6	2.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	298	PEG 3350, NaCl, pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 298K