2B10
Crystal Structure of the Protein-Protein Complex between F82S cytochrome c and cytochrome c peroxidase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2004-04-21 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 44.575, 113.448, 88.002 |
| Unit cell angles | 90.00, 104.55, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.800 |
| R-factor | 0.285 |
| Rwork | 0.284 |
| R-free | 0.30600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.018 |
| RMSD bond angle | 2.028 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.9999) |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298 | PEG 3350, NaCl, BOG, pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
